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KMID : 0043319980210060718
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Archives of Pharmacal Research
1998 Volume.21 No. 6 p.718 ~ p.722
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Hepatoprotective Activity of Scopoletin, a Constituent of Solanum lyratum
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Kang So-Young
Sung Sang-Hyun
Park Jong-Hee
Kim Young-Choong
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Abstract
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Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part of Solanum lyratum Thunb. by the activity-guided fractionation employing carbon te trachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1mcM to 50mcM. Further studies revealed that at the concentration of 10mcM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.
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KEYWORD
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Hepatoprotective activity, Scopolein, Solanum lyratum, Carbon tetrachloride, Primary cultured rate hepatocyte
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